Product Description

Features:

  • Multi-purpose, high purity
  • Enhanced resolution and clarity
  • Reduced background
  • Low EEO / increased electrophoretic mobility
  • RNase, DNase, Protease-free
  • High hysteresis, improved thermal stability

Benchmark Agarose LE is a highly purified agarose, suitable for a variety of molecular biology applications. It is refined using an advanced process that excludes the use of organic solvents, yielding a cleaner end-product with a significantly reduced environmental impact.Agarose LE can be used for analyses of proteins and nucleic acids of various sizes (150 bp to 6 kbp).
 
It's low EEO (< or = 0.13-mr) promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO also provides a reduction in band distortion (caused by counterflow) that  can result from the presence of excessive sulfate-rich negative ions.
 
Agarose LE is widely used for nucleic acid electrophoresis (analytical and preparative), protein electrophoresis (including radial immunodiffusion) and various blotting protocols.

It is easily soluble, free of nucleases, and easy to use. It is highly transparent (forms a clear, colorless solution at 1g:100ml H2O), and exhibits exceptionally low absorption of chemical staining agents. Pore size can be adjusted by simple modifications to the concentration ratio.

Formulated for high gel strength and integrity, Agarose LE exhibits exceptional thermal stability and mechanical resistance, insuring safe, easy handling, regardless of whether a denaturing agent has been added.

Technical Data:

  • Moisture Content: < or = 7%
  • EEO (electroendosmosis): < or = 0.13 - mr
  • Sulfate:< or = 0.20%
  • Gelling Temp. (at 1.5%): 36°C ± 1.5°C
  • Melting Temp. (at 1.5%): 88°C ± 1.5°C
  • Gel Strength (at 1.0%): 1200g/cm2
  • Gel Strength (at 1.5%): 2500g/cm2
  • DNase / RNase: None Detected
  • Protease: None Detected
  • Endonuclease: None Detected

Specifications

Specifications
Manufacturer Benchmark Scientific
Item Ships From New Jersey

Literature 1

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Returns

Request for return must be submitted to Customer Service Within 30 days of product shipment. All returns must be in pristine, brand-new, re-sellable condition and in their original packaging. A 15% restocking fee may be deducted at the discretion of the manufacturer. The manufacturer reserves the right to refuse all returns that are not complete with their original packaging. If an item has been used and is permanently marked and/or damaged in any way, return will not be accepted. All shipping charges remain payable by the purchaser.

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