Product Description

Features:

  • Multi-purpose, high purity
  • Enhanced resolution and clarity
  • Reduced background
  • Low EEO / increased electrophoretic mobility
  • RNase, DNase, Protease-free
  • High hysteresis, improved thermal stability

Benchmark Agarose LE is a highly purified agarose, suitable for a variety of molecular biology applications. It is refined using an advanced process that excludes the use of organic solvents, yielding a cleaner end-product with a significantly reduced environmental impact. Agarose LE can be used for analyses of proteins and nucleic acids of various sizes (150 bp to 6 kbp).
 
It's low EEO (< or = 0.13-mr) promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO also provides a reduction in band distortion (caused by counterflow) that  can result from the presence of excessive sulfate-rich negative ions.
 
Agarose LE is widely used for nucleic acid electrophoresis (analytical and preparative), protein electrophoresis (including radial immunodiffusion) and various blotting protocols.

It is easily soluble, free of nucleases, and easy to use. It is highly transparent (forms a clear, colorless solution at 1g:100ml H2O), and exhibits exceptionally low absorption of chemical staining agents. Pore size can be adjusted by simple modifications to the concentration ratio.

Formulated for high gel strength and integrity, Agarose LE exhibits exceptional thermal stability and mechanical resistance, insuring safe, easy handling, regardless of whether a denaturing agent has been added.

Technical Data:

  • Moisture Content: < or = 7%
  • EEO (electroendosmosis): < or = 0.13 - mr
  • Sulfate: < or = 0.20%
  • Gelling Temp. (at 1.5%): 36°C ± 1.5°C
  • Melting Temp. (at 1.5%): 88°C ± 1.5°C
  • Gel Strength (at 1.0%): 1200g/cm2
  • Gel Strength (at 1.5%): 2500g/cm2
  • DNase / RNase: None Detected
  • Protease: None Detected
  • Endonuclease: None Detected
  • Storage Conditions: Room Temperature

Specifications

Specifications
Manufacturer Benchmark Scientific
Item Ships From New Jersey

Literature

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Product Video

 

Returns

Products must be returned in unused, resalable condition with the original packaging. Please contact Customer Service for a valid return authorization within 30 days of receipt of purchase. A minimum restocking fee of 20% of the purchase price will be deducted from the refund. Customers original shipping charges will not be reimbursed. The customer is responsible for return shipping charges and insuring the package. Credit will not be issued if product is received damaged.

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