High Efficient Erlenmeyer Flask, PC, Wide-Mouth, Flat Bottom, 3000 mL

The High Efficient Wide-Mouth Erlenmeyer Flask, PC, Flat Bottom, 3000 mL is available with a plug seal or vent filter cap. It is a large-volume culture flask that allows cells to show strong viability with large expression amounts of proteins in the culture of mammalian cells and insect cells. During the culturing process, the use rate of the shaker is significantly increased, and the survival rate and viability of cells are both dramatically elevated. NEST culture flasks also provide high repeatability, which allows high inter-batch consistency of cell growth and yield.

Features:

• The flat-bottom bottle is made of medical-grade polycarbonate (PC) which has high transparency, great impact resistance, oxidation resistance, and can withstand a high temperature of up to 121°C
• Choice of plug seal cap or vent filter cap
• The vent filter cap is equipped with a 0.22 μm breathable film which is air-permeable and water-proof to efficiently prevent the passing of micro-organisms and contamination and to ensure gas exchange for good growth of cells or bacteria
• The scale on the flask body, made by injection molding, is clear and accurate when looked at from the outside, making it easier to observe the filled liquid volume of the culture medium
• The bottleneck diameter increased from 67 mm to 90 mm to increase oxygen flux and improve the efficiency of cell culture
• The wide-mouth neck design is convenient for sampling and liquid filling
• These flasks are optimized with lengthened necks that are convenient for customers to grip and can prevent the hand of the customer from being too close to the flask mouth when pouring liquid or holding the flask
• Additionally, they meet the aseptic operating practices of pharmaceutical companies
• The bottom is optimized to reduce the space occupation, significantly improving the use rate of the shaker, reducing the R&D and production costs of customers
• Suitable for suspension cell culture or bacterial culture and can also be used for medium preparation, mixing, and storage
• Individually packaged in sterile bags

Specifications:

• Sterilization: Yes
  • Sterilized by E-beam
  • Sterility Assurance Level: SAL = 10^-6
  • The product has been irradiated and dosimetrically released based on ANSI/AAMI/ISO 11137
  • All lots are sterilized and certified for proper dosage levels
• Materials: Meet USP, Class VI standards
  • Bottle: Polycarbonate (PC)
  • Plug Seal Cap: Polypropylene (PP)
  • Vent Filter Cap: Polypropylene (PP) cap with Polytetrafluoroethylene (PTFE) filter
• Non-Pyrogenic, DNase/RNase free
• BSE/TSE: These products are deemed animal-free
• Performance Testing: Each manufacturing lot is sampled and tested by standard operating procedures
  • Appearance Inspection: Qualified
  • Cell Culture Test: Qualified
  • Sealing Test: Qualified
  • Packaging inspection: Qualified
• Temperature Range: Stored at room temperature
• Shelf Life: 5 Years after the date of production

Recommendations:

• The amount of culture medium should be well-controlled, around 30%-40% of the total volume of the flask.
• For the control of the rotation speed during the cultivation process, it is recommended that the starting rotation speed is 75-125 rpm which can be adjusted in practice.
• Pay attention to the water level in a liquid-oscillating shaker or the temperature in an air-oscillating shaker.