Tetracycline Hydrochloride is an antibiotic that inhibits protein synthesis (elongation) by preventing the binding of amino-tRNA to ribosome. Tetracycline is for use in cell culture applications.
The tetracycline powder is used to make a stock solution at 5mg/ml in water. The working concentration for stringent plasmids is 10µg/ml and 50µg/ml for relaxed plasmids.
If dissolved in ethanol, the antibiotic solution need not be sterilized.
Magnesium ions are antagonists of tetracycline. Use media without magnesium salts (e.g. LB medium) for the selection of bacteria resistant to tetracycline.
Molecular Weight: 480.90
Molecular Formula: C22H24N2O8HCl
Potency: Min. 900ug/mg
pH (1% Water): 2.3 ± 0.5
Moisture: Max. 2.0%
4-Ephianhydrotetracycline: Max. 2.0%
Heavy Metals: Max. 0.005
Appearance: Yellow powder
Molecular Biology Specifications:
Antibiotic Inhibition/Sensitivity Assay: Pass
Storage: Store at +4°C. Warm to room temperature prior to use. Keep tightly sealed. Store Tetracycline Hydrochloride solution in a light-tight container at -20°C.
Warning: Poison. Federal law prohibits dispensing without prescription. Not for use in humans. Not for drug use. Avoid contact with eyes, skin and clothing. Avoid breathing dust. Wash thoroughly with soap and water after handling. See Material Safety Data Sheet for additional information.
Please Note: This item is manufactured for laboratory research only and will not be shipped to other types of commercial or residential addresses.
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