Ponceau S is a stain used to visualize proteins on nitrocellulose or PVDF membranes.
To create Ponceau S solution; dissolve 0.5gm of Ponceau S in 1ml of glacial acetic acid, and bring to 100ml with ddi water.
To stain with Ponceau S, remove blot and rinse with ddi water. Then, stain with Ponceau S solution for 1 to 2 minutes. Rinse for 3 to 5 minutes with ddi water. Red bands should appear against the white background.
To reversal stain with Ponceau S Solution, place the nitrocellulose membrane in Ponceau S solution for 5 minutes at room temperature. Destain in ddi water for 2 minutes and mark the molecular weight standard with indelible ink. To complete the destain, soak in water for 10 minutes. Alternatively, use a 0.1M NaOH solution for 5 minutes if proteins of interest are not basic.
Molecular Formula: C22H12N4Na4O13S4
Molecular Weight: 760.61
Em (520nm, Water): ≥ 27,000
Loss on Drying: ≤ 6%
Solubility (0.1%, Water): Pass
Appearance: Deep red, crystalline powder
Molecular Biology Specifications:
DNase assay: None Detected
RNase assay: None Detected
Protease assay: None Detected
Storage: Store at room temperature. Keep tightly sealed. Protect from moisture.
Warning: Irritant. Toxic. Toxic by inhalation. May be harmful or fatal if swallowed. Avoid breathing vapors. Avoid contact with eyes, skin, and clothing. Wash thoroughly with water after handling. See Material Safety Data Sheet for additional information.
Please Note: This item is manufactured for laboratory research only and will not be shipped to other types of commercial or residential addresses.
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