Product Description

Applications: Methylene Blue is a recommended stain for agarose gels when recovery of DNA is required. The benefit of using methylene blue (rather than ethidium bromide) is the ability to use a white light box for visualization and photography in place of a UV light box. Using a white light box eliminates mutations that may affect the integrity of the isolated DNA.

Staining DNA Protocol:

  1. Stain a 1% gel for 15 minutes with 0.02% Methylene Blue Solution in distilled water.
  2. Destain with distilled water for 15 minutes.

Staining RNA Protocol (bonded to nylon or nitrocellulose membranes):

  1. Soak the dried filter in 5% acetic acid at room temperature for 15 minutes.
  2. Place the filter in 0.5M sodium acetate (pH 5.2) and 0.04% methylene blue for 5 -10 minutes at room temperature.
  3. Rinse the filter in water for 5 - 10 minutes.

Purity of Methylene Blue: The purity of methylene blue is stated as Dye Content as measured on a dry basis. The Dye Contenet for IBI Methylene BLue is 98% - 103%.

Physical Specifications:

  • CAS#: 7220-79-3
  • Molecular Formula: C16H18ClN3S x 3H2O
  • Molecular Weight: 373.9
  • Appearance:Iridescent green-black powder
  • Em (661nm water): >69,000
  • Residue on Ignition: <1.0%
  • Arsenic: <0.0008%
  • Copper: <0.01%
  • Moisture: Max. 15.0%
  • Zinc: <0.001%
  • Lead: <0.001%
  • Dye Content (Dry Basis): 98 - 103%

Molecular Biology Specifications:

  • DNase assay: None Detected
  • RNase assay: None Detected
  • Protease assay: None Detected

Storage: Store at room temperature. Protect from moisture.

Warning: Irritant. Avoid contact with eyes, skin, and clothing. Do not take internally. Wash thoroughly with water after handling. See Material Safety Data Sheet for additional information.


Please Note: This item is manufactured for laboratory research only and will not be shipped to other types of commercial or residential addresses.


Manufacturer IBI Scientific
Item Ships From Iowa

Literature 1

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