Applications: Methylene Blue is a recommended stain for agarose gels when recovery of DNA is required. The benefit of using methylene blue (rather than ethidium bromide) is the ability to use a white light box for visualization and photography in place of a UV light box. Using a white light box eliminates mutations that may affect the integrity of the isolated DNA.
Staining DNA Protocol:
Stain a 1% gel for 15 minutes with 0.02% Methylene Blue Solution in distilled water.
Destain with distilled water for 15 minutes.
Staining RNA Protocol (bonded to nylon or nitrocellulose membranes):
Soak the dried filter in 5% acetic acid at room temperature for 15 minutes.
Place the filter in 0.5M sodium acetate (pH 5.2) and 0.04% methylene blue for 5 -10 minutes at room temperature.
Rinse the filter in water for 5 - 10 minutes.
Purity of Methylene Blue: The purity of methylene blue is stated as Dye Content as measured on a dry basis. The Dye Contenet for IBI Methylene BLue is 98% - 103%.
Molecular Formula: C16H18ClN3S x 3H2O
Molecular Weight: 373.9
Appearance:Iridescent green-black powder
Em (661nm water): >69,000
Residue on Ignition: <1.0%
Moisture: Max. 15.0%
Dye Content (Dry Basis): 98 - 103%
Molecular Biology Specifications:
DNase assay: None Detected
RNase assay: None Detected
Protease assay: None Detected
Storage: Store at room temperature. Protect from moisture.
Warning: Irritant. Avoid contact with eyes, skin, and clothing. Do not take internally. Wash thoroughly with water after handling. See Material Safety Data Sheet for additional information.
Please Note: This item is manufactured for laboratory research only and will not be shipped to other types of commercial or residential addresses.
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