The MaGELin Western Transfer Protein System allows rapid transfer of protein or peptide samples or nucleic acids separated by mini-polyacrylamide gels onto 8cm x 10cm or 10cm x10cm membranes.
The unit accommodates two cassette sandwiches for simultaneous transfer of two gels. Proteins separated by mini PAGE gels can be transferred within the electrode module by high-intensity electrical fields, while maintaining equal resolution. Depending on the molecular weight and sequence of the protein/peptide, the transfer should take 60 to 90 minutes.
The unit consists of two hinged, perforated transfer cassettes, each color-coded with 1/8" acrylic and a cassette lock. The cassettes slide into an electrode module. The distance between the electrodes is 4.5cm. The blotting application is dependent on the voltage applied over the distance between the electrodes. By keeping the distance between the electrodes at a minimum, higher electrical fields are produced, thus allowing a more efficient protein transfer.
The patented vented lid keeps the buffer cool during transfer; for longer runs, a stir bar can be added to recirculate the buffer, thereby eliminating heat gradients during the transfer. In extreme cases, our IBI ice-pack can be inserted into the tank for even better cooling.
Buffer chamber is constructed of heavy 1/4" cast acrylic and has a maximum buffer volume of 1200 ml.
Comes with: Patented Vented Lid, Buffer Tank, Western Transfer Module, Filter Pads, Power Cords, Operating Manual and 4-year limited warranty.
Unit Dimensions: 13.3cm (H) x 15cm (W) x 16cm (L)
Buffer Capacity: 1200ml
Maximum Gel Size: 10cm x 10cm
Voltage Limit: 100VDC
Current Limit: 500mA
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