Product Description

Applications:

  • Kanamycin is a broad spectrum aminoglycoside antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in cell culture media for the isolation of Group D Streptococci on Kanamycin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin medium. Kanamycin sulfate can also be used as selection agent for cells transformed with kanamycin B resistance gene.
  • Kanamycin acts by binding to the 70S ribosomal subunit, inhibiting translocation.
  • Kanamycin sulfate is effective against gram negative and gram positive bacteria, as well as mycoplasma.

Stock Solution:

  • A typical stock solution is 10mg/ml in water.
  • Solution should sterilized via filtration through a 0.22 micron filter.
  • A working concentration for stringent plasmids is 10ug/ml and 50ug/ml for relaxed plasmids.

Physical Specifications:

  • CAS#: 25389-94-0
  • Formula Weight: 582.58
  • Molecular Formula: C18H36N4O11H2SO4
  • Potency: >750mcg/mg
  • pH (1% water): 6.5 - 8.5
  • Moisture: Max. 4.0%
  • Appearance: Fine white to off-white powder

Molecular Biology Specifications:

  • Antibiotic Inhibition/Sensitivity Assay: Pass
  • Crystallinity: Pass
  • Chromatographic Purity: Pass
  • Kanamycin Sulfate is derived via microbial fermentation. No animal products or by-products are used in the manufacturing process or the fermentation media.

Storage: Store at +4oC. Warm to room temperature before opening. Keep tightly sealed. Store Kanamycin Sulfate solution in a light-tight container at -20oC.

Warning: Federal law prohibits dispensing without prescription. Not for use in humans. Not for drug use. Sensitizer/Irritant/Suspected Teratogen. Avoid contact with eyes, skin and clothing. Avoid breathing dust. Wash thoroughly with soap and water after handling. See Material Safety Data Sheet for additional information.

 

Please Note: This item is manufactured for laboratory research only and will not be shipped to other types of commercial or residential addresses.

Specifications

Specifications
Manufacturer IBI Scientific
Item Ships From Iowa

Literature 1

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