Product Description

Applications: IPTG (Isopropyl -D-thiogalatopyranoside) is an inducer of β-galactosidase activity in E. coli. It is commonly used in conjunction with X-GAL to detect lac gene expression in cloning applications to allow dection of recombinant molecules.

Physical Specifications:

  • CAS#: 367-93-1
  • Molecular Formula: C9H18O5S
  • Formula Weight: 238.31
  • Purity: Min. 99.0%
  • Melting Point: 110-114oC
  • IR (KBr): Conforming as Standard
  • Dioxane (GLC): < 1ppm
  • Appearance: White crystalline powder

Molecular Biology Specifications:

  • Colorimetric Transformation Assay: Pass

Protocol For Stock Solution: Prepare a stock solution of IPTG at 0.1M (23.8 mg/mL) in sterile distilled water and filter sterilize through a 0.22 micron disposable filter. To either of the below solutions add 3ml/L IPTG stock solution. When pouring plates add 15gm bacto-agar per liter.

Solution1: 10X M9 Salts Solution:

60g Na2HPO4

30g KH2PO4

5g NaCl

10g NH4Cl

Adjust the pH to 7.4, add water to a final volume of 1L and autoclave.

For M9 media add:

100ml 10X M9 Salt Solution

1ml 0.01M CaCl2

2ml 1.0M MgSO4

Adjust to a final volume of 1 liter with distilled water.

Supplement with (optional):

0.2% Casamino Acids

0.4% Glucose

40 mg/ml Thiamine-HCL

Solution2: 2X YT Media:

16g/L Tryptone

10g/L Yeast Extract

5g/L NaCl

Storage: Store dessicated at - 20oC. Warm to room temperature before opening. It is usable at room temperature for 36 hours. Keep tightly sealed.

Warning: Irritant. Avoid contact with eyes, skin, and clothing. Avoid breathing dust. Wash thoroughly with water after handling. See Material Safety Data Sheet for additional information.

 

Please Note: This item is manufactured for laboratory research only and will not be shipped to other types of commercial or residential addresses.

Specifications

Specifications
Manufacturer IBI Scientific
Item Ships From Iowa

Literature 1

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(Size: 40.8 KB)

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