Applications: IPTG (Isopropyl -D-thiogalatopyranoside) is an inducer of β-galactosidase activity in E. coli. It is commonly used in conjunction with X-GAL to detect lac gene expression in cloning applications to allow dection of recombinant molecules.
Molecular Formula: C9H18O5S
Formula Weight: 238.31
Purity: Min. 99.0%
Melting Point: 110-114oC
IR (KBr): Conforming as Standard
Dioxane (GLC): < 1ppm
Appearance: White crystalline powder
Molecular Biology Specifications:
Colorimetric Transformation Assay: Pass
Protocol For Stock Solution: Prepare a stock solution of IPTG at 0.1M (23.8 mg/mL) in sterile distilled water and filter sterilize through a 0.22 micron disposable filter. To either of the below solutions add 3ml/L IPTG stock solution. When pouring plates add 15gm bacto-agar per liter.
Solution1: 10X M9 Salts Solution:
Adjust the pH to 7.4, add water to a final volume of 1L and autoclave.
•For M9 media add:
100ml 10X M9 Salt Solution
1ml 0.01M CaCl2
2ml 1.0M MgSO4
Adjust to a final volume of 1 liter with distilled water.
•Supplement with (optional):
0.2% Casamino Acids
40 mg/ml Thiamine-HCL
Solution2: 2X YT Media:
10g/L Yeast Extract
Storage: Store dessicated at - 20oC. Warm to room temperature before opening. It is usable at room temperature for 36 hours. Keep tightly sealed.
Warning: Irritant. Avoid contact with eyes, skin, and clothing. Avoid breathing dust. Wash thoroughly with water after handling. See Material Safety Data Sheet for additional information.
Please Note: This item is manufactured for laboratory research only and will not be shipped to other types of commercial or residential addresses.
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