Product Description

Applications: IPTG (Isopropyl -D-thiogalatopyranoside) is an inducer of β-galactosidase activity in E. coli. It is commonly used in conjunction with X-GAL to detect lac gene expression in cloning applications to allow dection of recombinant molecules.

Physical Specifications:

  • CAS#: 367-93-1
  • Molecular Formula: C9H18O5S
  • Formula Weight: 238.31
  • Purity: Min. 99.0%
  • Melting Point: 110-114oC
  • IR (KBr): Conforming as Standard
  • Dioxane (GLC): < 1ppm
  • Appearance: White crystalline powder

Molecular Biology Specifications:

  • Colorimetric Transformation Assay: Pass

Protocol For Stock Solution: Prepare a stock solution of IPTG at 0.1M (23.8 mg/mL) in sterile distilled water and filter sterilize through a 0.22 micron disposable filter. To either of the below solutions add 3ml/L IPTG stock solution. When pouring plates add 15gm bacto-agar per liter.

Solution1: 10X M9 Salts Solution:

60g Na2HPO4

30g KH2PO4

5g NaCl

10g NH4Cl

Adjust the pH to 7.4, add water to a final volume of 1L and autoclave.

For M9 media add:

100ml 10X M9 Salt Solution

1ml 0.01M CaCl2

2ml 1.0M MgSO4

Adjust to a final volume of 1 liter with distilled water.

Supplement with (optional):

0.2% Casamino Acids

0.4% Glucose

40 mg/ml Thiamine-HCL

Solution2: 2X YT Media:

16g/L Tryptone

10g/L Yeast Extract

5g/L NaCl

Storage: Store dessicated at - 20oC. Warm to room temperature before opening. It is usable at room temperature for 36 hours. Keep tightly sealed.

Warning: Irritant. Avoid contact with eyes, skin, and clothing. Avoid breathing dust. Wash thoroughly with water after handling. See Material Safety Data Sheet for additional information.


Please Note: This item is manufactured for laboratory research only and will not be shipped to other types of commercial or residential addresses.


Manufacturer IBI Scientific
Item Ships From Iowa

Literature 1

(Size: 40.8 KB)


If you would like to return an item, please contact customer service within 30 days of product shipment to receive a return authorization (RA) number. All returns must include the RA number, include original packaging and be shipped pre-paid. Items returned without proper authorization will not receive credit. A 15-25% restocking charge may be assessed for returned items. All shipping charges remain payable.


Ask a Question

Customer Questions
This product has no questions.
Did you find what you were looking for?