Applications: Used in hybridization, electrophoresis applications, and to denature DNA and RNA
Spectral Grade Formamide is used as a denaturant in hybridization and is used in hybridization buffers made from standard recipes. It separates the two strands of the double helix. Can also be used as a cryoprotectant and gel stabilizer. Ideal for preparing brain sections of mice for BrdU immunohistochemistry and FISH analysis of HeLa cells.
IBI Formamide is supplied in an amber glass bottle, it is distilled, deionized, and packaged under nitrogen to prevent oxidation. Bottles that have been opened and stored should be deionized prior to use by mixing 1 gm of mixed bed resin per 10 ml of formamide for 30 minutes. It is a clear liquid amide derived from formic acid.
Purity is determined by way of gas chromatography.
Formula Weight: 45.04
Molecular Formula: CH3NO
Appearance: Clear, viscous liquid
Purity: Min. 99.0%
Conductivity: Max. 100µmho
Abs. @280: Max. 0.08
Molecular Biology Specifications:
DNase assay: None Detected
RNase assay: None Detected
Protease assay: None Detected
Storage: Store at +4°C in the dark. Warm to room temperature prior to opening.
Warning: Irritant/Lachrymator/Suspected mutagen. Avoid breathing vapors. Use with adequate ventilation. Avoid contact with eyes, skin and clothing. If contact should occur, wash with copious amounts of water. See Material Safety Data Sheet for additional information.
Please Note: This item is manufactured for laboratory research only and will not be shipped to other types of commercial or residential addresses.
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