Ethidium Bromide Solution can be used to detect both single and double stranded nucleic acids (both DNA and RNA). However, Ethidium bromide ( EtBr ) does not intercollate into single-stranded DNA very well; therefore, the fluorescence of single-stranded DNA is relatively poor in comparison to double-stranded DNA.
Use 2.5 µl of ethidium bromide solution for every 50 ml of molten agarose, running buffer, or staining solution which is about one drop.
The recommended final concentration of Ethidium Bromide in an agarose gel and/or electrophoresis buffers is 0.5µg/ml.
How do I make a stock solution with a concentration 0.5 µg/ml? Add one drop (approximately 50 µl) of IBI 10 mg/ml Ethidium Bromide Solution to 1-liter of DDI water to make a stock solution with a desired concentration of 0.5 µg/ml. This stock solution of ethidium bromide can then be added to the researcher’s agarose or buffer solution.
Molecular Formula: C21H20BrN3
Molecular Weight: 394.32
Appearance: Dark red haze-free liquid
Purity: Min. 98.0%
Moisture: Max. 8.0%
Solution Concentration: 10mg per ml
Molecular Biology Specifications:
DNase assay: None Detected
RNase assay: None Detected
Storage: Store at room temperature. Keep tightly sealed. Protect from moisture.
Warning: Poison/Suspected mutagen. Avoid contact with eyes, skin and clothing. Avoid breathing dust. Do not take internally. Wash thoroughly with water after handling. See Material Safety Data Sheet for additional information.
Please Note: This item is manufactured for laboratory research only and will not be shipped to other types of commercial or residential addresses.
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