Chloramphenicol is a broad spectrum antibiotic that is active against a wide variety of gram positive and gram negative bacteria, including most anaerobic organisms. This antibiotic blocks bacterial protein synthesis by inhibiting the peptidyl transferase activity of the 50S ribosomal subunit (elongation inhibition).
Chloramphenicol is used in the amplification of plasmids and colony hybridizations.
The antibiotic powder is used to make a stock solution at 34mg/ml in ethanol. The working concentration for stringent plasmids is 25µg/ml and 170µg/ml for relaxed plasmids.
If dissolved in ethanol, the chloramphenicol solution need not be sterilized.
Molecular Weight: 323.13
Molecular Formula: C11H12Cl2N2O5
Melting Point: 149 - 153°C
pH (2.5% Water): 6.0 ± 1.5
Purity: 97.0 - 103.0%
Appearance: White to off-white powder
Molecular Biology Specifications:
Antibiotic Inhibition/Sensitivity Assay: Pass
Chromatographic Purity: Pass
Storage: Store at +4°C. Warm to room temperature prior to use. Keep tightly sealed. Store Chloramphenicol solution in a light-tight container at -20°C.
Warning: Poison. Suspected carcinogen. Federal law prohibits dispensing without prescription. Not for use in humans. Not for drug use. Avoid contact with eyes, skin and clothing. Avoid breathing dust. Wash thoroughly with soap and water after handling. See Material Safety Data Sheet for additional information.
Please Note: This item can only be shipped to a clinical or research facility; orders shipping to a residential address will be cancelled.
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