The 5X RNA Gel Loading Kit contains reagents for denaturing and loading RNA samples onto a formaldehyde gel using MOPS as a buffer. The reagents are nuclease free and contain ultra pure deionized formamide and DEPC water.
The RNA sample is dissolved in 10µl of DEPC water and mixed with 35µl of denaturing solution. Heat the sample to 65°C for 5 minutes. Once the solution has cooled, add 5µl of loading dye. The sample is now ready to load into the gel.
Molecular Formula: Not Applicable
Molecular Weight: Not Applicable
Molecular Biology Specifications:
DNase assay: None Detected
RNase assay: None Detected
Storage: Store at +4°C. Warm to room temperature prior to opening. Keep tightly sealed, Protect from moisture.
Warning: Irritant. Avoid contact with eyes, skin and mucous membranes. Avoid breathing vapors. Wash thoroughly with water after handling. See Material Safety Data Sheet for additional information.
Please Note: This item is manufactured for laboratory research only and will not be shipped to other types of commercial or residential addresses.
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